RNF167 Knockout cell line (Hep G2)

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RNF167 Knockout cell line (Hep G2) Order

RNF167 Knockout cell line (Hep G2)
Catalog#
YKO-HT6996
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RNF167 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RNF167 Knockout cell line (Hep G2)
Species
Human
Gene Name
RNF167
Gene ID
26001
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript RNF167-201)
Validation: PCR+Sanger Sequencing
RNF167 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RNF167 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RNF167 gene knockout RNF167 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RNF167 Knockout cell line (Hep G2)
RNF167 Knockout cell line (Hep G2)
Catalog#
YKO-HT6996
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • RNF167 Knockout cell line (HeLa)

    RNF167 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11428
    YKO-HS11428(HeLa)
  • RNF167 Knockout cell line (HCT 116)

    RNF167 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11427
    YKO-HS11427(HCT 116)
  • RNF167 Knockout cell line (HEK293)

    RNF167 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11426
    YKO-HS11426(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • RNF167 Knockout cell line (HeLa)

    RNF167 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11428
      inquiry   More details
  • RNF167 Knockout cell line (HCT 116)

    RNF167 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11427
      inquiry   More details
  • RNF167 Knockout cell line (HEK293)

    RNF167 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11426
      inquiry   More details

RNF167 Knockout cell line (Hep G2) Order

RNF167 Knockout cell line (Hep G2)
Catalog#
YKO-HT6996
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RNF167 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RNF167 Knockout cell line (Hep G2)
Species
Human
Gene Name
RNF167
Gene ID
26001
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript RNF167-201)
Validation: PCR+Sanger Sequencing
RNF167 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RNF167 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RNF167 gene knockout RNF167 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RNF167 Knockout cell line (Hep G2)
RNF167 Knockout cell line (Hep G2)
Catalog#
YKO-HT6996
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • RNF167 Knockout cell line (HeLa)

    RNF167 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11428
    YKO-HS11428(HeLa)
  • RNF167 Knockout cell line (HCT 116)

    RNF167 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11427
    YKO-HS11427(HCT 116)
  • RNF167 Knockout cell line (HEK293)

    RNF167 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11426
    YKO-HS11426(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • RNF167 Knockout cell line (HeLa)

    RNF167 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11428
      inquiry   More details
  • RNF167 Knockout cell line (HCT 116)

    RNF167 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11427
      inquiry   More details
  • RNF167 Knockout cell line (HEK293)

    RNF167 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: RNF167
    Gene ID: 26001
    Size: 1*10^6
    Catalog#: YKO-HS11426
      inquiry   More details

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